Project Update

Spring Semester Wrap-up! Aeromonas update

As the spring semester wraps up and the 2018-19 school year comes to a close. I am currently prepping for the LAST field season of my doctoral work! I am slated to graduate in May 2020, which means I will be defending my dissertation in February or March, most likely. I wanted to create a quick video, just so you all could get an idea of where we are with our systems model for the Aeromonas hydrophila pathogen.

Catalpa Creek Tributaries - A Preliminary Investigation

You can download a PDF copy of a recent presentation on the topic of this post here At this point, we have completed the lab work-up of nearly all of the 2018 macroinvertebrate coarse samples from the two Catalpa Creek tributaries that we are investigating. I have been working through some preliminary analyses to get an idea of what if going on the two streams. So far, the analyses include simple community indices including species richness, diversity, and evenness, and a Canonical Correlation Analysis (“CCA”).

Preparing for Prelims!

Well, the time has officially arrived! Next week, I will begin my preliminary exams to fulfill my requirements to move from a Ph.D student to a Ph.D candidate! This means that starting on Monday, I will start a week of written exams, one from each of my dissertation committee members. Over the last 2 months or so, I have been studying every aspect of biology, ecology, immunology, and aquaculture that relates to my research.

Genotype Genocide??

As mentioned in the project description of my Aeromonas work, we are currently working with two different strains/genotypes of virulent Aeromonas hydrophila. Dealing with multiple strains of a bacteria can prove difficult with creating vaccines or administering antibiotics so we wanted to see how the temporal distribution of these two strains compared. So, we analyzed the isolates from various cases using polymerase chain reaction (PCR) and electrophoresis. The image below shows what a typical electrophoresis gel looks like and how to read it.